High Performance Liquid Chromatography (HPLC)
Shimadzu LC-20AD Liquid Chromatograph
The system is equipped with two binary solvent pumps, a built-in degasser, a column oven, an autosampler, and is controlled by LabSolutions Workstation software.
We also have a Pierce Reacti-Therm heating module for evaporating solvents as part of the sample preparation steps.
Methods Currently Available
Mycophenolic Acid & Mycophenolic Acid Glucuronide (MPA and MPAG)
Using a method modified from published literature, we are able to quantify mycophenolic acid and its glucuronide derivative from just 200 µL of plasma. This is currently being used for a pharmacogenomics/pharmacokinetics research study for Dr. Laneshia Tague. The AMR for MPA is 0.06 – 50.0 mg/L and for MPAG, 0.035 – 200 mg/L.
ATP, ADP, and AMP
Using an isocratic method with a polar C18 column, we are able to separate and quantify the nucleotides ATP, ADP, and AMP. We are currently performing this analysis for Dr. David Sibley’s group and their research studies of protozoan parasites. Our analytical measuring range is 0.10 – 100 µM. Sample injection volume is 15 – 30 µL.
Method Development
If you have a planned study with analytes that may be amenable to HPLC quantitation, please contact Dr. Jennifer Powers Carson (powers.jennifer.l@wustl.edu) to discuss feasibility and time frame of your study.
Cost will vary depending on how much method development is required and how many samples are anticipated.